{"id":234,"date":"2022-10-12T14:30:00","date_gmt":"2022-10-12T06:30:00","guid":{"rendered":"https:\/\/www.helixmatrix.net\/?p=234"},"modified":"2022-11-12T14:58:06","modified_gmt":"2022-11-12T06:58:06","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e6%95%b0%e6%8d%ae%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"https:\/\/www.helixmatrix.net\/index.php\/2022\/10\/12\/%e5%8d%95%e7%bb%86%e8%83%9e%e6%95%b0%e6%8d%ae%e5%88%86%e6%9e%90\/","title":{"rendered":"\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790"},"content":{"rendered":"\n<p>\u5355\u7ec6\u80de\u6d4b\u5e8f\u8ba9\u6211\u4eec\u80fd\u591f\u5728\u524d\u6240\u672a\u6709\u7684\u7cbe\u5ea6\u6c34\u5e73\u4e0b\u7814\u7a76\u57fa\u56e0\u8868\u8fbe\uff0c\u8be5\u6280\u672f\u540c\u65f6\u884d\u751f\u4e86\u4f17\u591a\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u65b9\u6cd5\u3002\u76ee\u524d\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u65b9\u6cd5\u7e41\u591a\uff0c\u4ece\u8fd9\u4e9b\u65b9\u6cd5\u4e2d\u6311\u9009\u9002\u7528\u4e8e\u7528\u6237\u81ea\u5df1\u7684\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u65b9\u6cd5\uff0c\u6210\u4e3a\u4e86\u5927\u5bb6\u7684\u5173\u6ce8\u70b9\u3002<\/p>\n\n\n\n<p>\u9664\u4e86\u76ee\u524d\u5e38\u7528\u7684\u5206\u6790\u6280\u672f\u5916\uff0c\u5355\u7ec6\u80de\u5206\u6790\u8fd8\u5728\u4e0d\u65ad\u6d8c\u73b0\u65b0\u7684\u6539\u8fdb\u7b97\u6cd5 \uff0c\u5982Cell BLAST \u53d8\u5206\u81ea\u7f16\u7801\u5668 \uff0cscDSC\u805a\u7c7b\u7b97\u6cd5\u7b49\uff0c\u87ba\u65cb\u77e9\u9635\u5b9e\u73b0\u4e86\u76ee\u524d\u5e02\u9762\u4e0a\u5e38\u89c1\u7684\u7b97\u6cd5\u5e93\uff0c\u4ee5\u53ca\u81ea\u4e3b\u6539\u8fdb\u7b97\u6cd5\u5e93\uff0c\u52a9\u529b\u5355\u7ec6\u80de\u5206\u6790\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img decoding=\"async\" loading=\"lazy\" width=\"550\" height=\"449\" src=\"https:\/\/www.helixmatrix.net\/wp-content\/uploads\/2022\/11\/68747470733a2f2f64726976652e676f6f676c652e636f6d2f75633f6578706f72743d766965772669643d31596f58334638674e4748354b3041467534774664643563634558542d6d505f4a.png\" alt=\"\" class=\"wp-image-235\" srcset=\"https:\/\/www.helixmatrix.net\/wp-content\/uploads\/2022\/11\/68747470733a2f2f64726976652e676f6f676c652e636f6d2f75633f6578706f72743d766965772669643d31596f58334638674e4748354b3041467534774664643563634558542d6d505f4a.png 550w, https:\/\/www.helixmatrix.net\/wp-content\/uploads\/2022\/11\/68747470733a2f2f64726976652e676f6f676c652e636f6d2f75633f6578706f72743d766965772669643d31596f58334638674e4748354b3041467534774664643563634558542d6d505f4a-300x245.png 300w\" sizes=\"(max-width: 550px) 100vw, 550px\" \/><\/figure>\n\n\n\n<ul>\n<li>Pre-processing(10x: cellranger)<\/li>\n\n\n\n<li>Quality control (filter\/doublets)<\/li>\n\n\n\n<li>Normalization<\/li>\n\n\n\n<li>Data correction and integration<\/li>\n\n\n\n<li>Feature selection<\/li>\n\n\n\n<li>dimensionality reduction and visualization<\/li>\n\n\n\n<li>Cluster analysis &amp; Annotation<\/li>\n\n\n\n<li>Compositional analysis<\/li>\n\n\n\n<li>Trajectory analysis<\/li>\n\n\n\n<li>Gene-level analysis (DEGs, Enrichment, GRN)<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5355\u7ec6\u80de\u6d4b\u5e8f\u8ba9\u6211\u4eec\u80fd\u591f\u5728\u524d\u6240\u672a\u6709\u7684\u7cbe\u5ea6\u6c34\u5e73\u4e0b\u7814\u7a76\u57fa\u56e0\u8868\u8fbe\uff0c\u8be5\u6280\u672f\u540c\u65f6\u884d\u751f\u4e86\u4f17\u591a\u5355\u7ec6\u80de\u6570\u636e\u5206\u6790\u65b9\u6cd5\u3002\u76ee\u524d\u5355\u7ec6\u80de\u6570\u636e [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":235,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5],"tags":[],"_links":{"self":[{"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/posts\/234"}],"collection":[{"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/comments?post=234"}],"version-history":[{"count":3,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/posts\/234\/revisions"}],"predecessor-version":[{"id":240,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/posts\/234\/revisions\/240"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/media\/235"}],"wp:attachment":[{"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/media?parent=234"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/categories?post=234"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.helixmatrix.net\/index.php\/wp-json\/wp\/v2\/tags?post=234"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}